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      ProfiBlot™ blot processors

      For automated processing of Western blot and Southern blot assay strips

      Tecan’s family of blot processors support automated washing and incubation of up to 48 assay strips per run. It includes the temperature controlled ProfiBlot T48, which takes care of hybridization and stringent wash steps, enabling walkaway processing of Southern blot assay strips. In addition, the ProfiBlot 48 takes care of non-heated incubation and washing steps for automated processing of Western blot assays strips.
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      Request pricing Talk to an expert




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      Tab 01 / Overview
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      Ease of use

      The ProfiBlot 48 and ProfiBlot T48 blot processors are easy to operate, and can be programmed either from a PC using the BlotWare™ software, or by using the integrated display and keypad. Dispense volumes, selection of dispense channels, shaking speed for incubation steps and wash parameters can be arranged in any combination within a protocol, and up to 20 different methods can be stored. Once programming and downloading are completed, the PC can be disconnected and the ProfiBlot will perform the assays in standalone mode.

      After the user has placed the assay strips into the individual wells of the tray, the samples can be added using a pipette. At the push of a button, the entire procedure can be performed with no further input from the user. The display provides a way of checking every stage of the process, and an audible signal indicates when the assay is complete.

      Delivering reliable results

      The reagent delivery system provides consistent results.

      For fast dispensing, the ProfiBlot processors have two reagent channels with three pumps working in parallel, and up to five channels for single position reagent addition. For fast aspiration of the wells, liquid is removed from the tray by using three aspiration pumps in parallel, processing three strips at a time. This fast method of dispensing and aspiration ensures reproducible timing, even for kits requiring short incubation times. Additionally, an automated cleaning procedure simplifies daily maintenance at the push of a button.

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      Automated processing 48 samples per run

      Load up to 48 assay strips into the individual wells of the tray, add the samples and press start.

      The ProfiBlot 48 and T48 instruments use disposable trays only, which are designed to meet the IVD-directive 98/79/EC and eliminate the need for tedious cleaning of the sample holders. This is advantageous over reusable sample holders, which are often difficult to clean due to the background color of the tray material, reducing chances of false positive results and a need for retesting.

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      Tab 02 / ProfiBlot 48
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      ProfiBlot 48

      Fast Western blot processor for automated washing and incubation of strip-based assays

      The ProfiBlot 48 provides reliability and increased productivity by automating all key protocol steps of Western blot processing, including time-consuming washing and incubation procedures.

      For optimum throughput, the ProfiBlot 48 processes up to 48 samples per run and supports a range of Western blot applications, such as for the confirmation of infectious and autoimmune diseases, as well as for allergy screening.

      The ProfiBlot 48 has been designed to improve safety and reliability in the laboratory, and to meet the IVD-directive 98/79/EC for Europe. It processes the entire assay in a disposable tray within an enclosed environment to minimize human exposure to potentially infectious samples.

      Product highlights include:

      • Automated processing of up to 48 samples per run
      • Fast washing of three assay strips in parallel for high throughput
      • Controlled timing for short incubation steps
      • On-board operation and BlotWare™ software for easy programming
      • Color-coded reagent delivery system for up to seven fluids
      • Reagent-saving feature minimizes dead volumes and running costs
      • Auto-calibration of dispense volumes eliminates tedious manual procedure
      • Separate collection of toxic and biohazardous waste minimizes waste treatment costs
      • Predefined cleaning protocols for easy maintenance
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      Specifications

      General
           
      Display unit Liquid crystal display with 2 rows of 16 digits
      Keyboard Four key membrane keyboard
      Number of dispensing channels 6 (optional 7)
      Capacity of reagent bottles 5 (optional 6) x 250 ml, 1 x 1000 ml
      Capacity of waste bottles 1 x 500 ml toxic waste , 1 x 2500 ml hazardous waste
      Program editor software Blotware™
      Computer interface USB, RS232
      Disposable 48 well tray Black, antistatic
      Variable
           
      No. of strips to be processed Up to 48 strips
      No. of processing steps for each program Up to 59 different steps in one program
      No. of programs Up to 20 different programs
      Dispense volume 250 µl to 3000 µl in 250 µl steps
      Incubation time 1 – 999 min in 1 min steps
      Dispensing accuracy @2000 µl: less than or equal to 10 %
      @250 µl: +25% to -20%
      Physical
           
      Width closed / openend 804 mm / 804 mm
      Height closed / openend 460 mm / 671.4 mm
      Depth closed/ opened 459 mm/ 510.6 mm
      Weight 36.8 kg
      Power
           
      Supply Auto-sensing for the settings
      100 – 120 V and 220 – 240 V
      50 or 60 Hz
      Main fuses 2 x T1 A / 250V (Slow Blow)
      Consumption Max. 85 VA

      Literature

      Brochure - ProfiBlot 48

      The ProfiBlot 48 is an automated Western Blot Processor for up 48 samples per run.

      Poster - ProfiBlot 48

      User story with Mikrogen, explaining how the ProfiBlot 48 can be used for a strip-based Western Blot assay.




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      Tab 03 / ProfiBlot 48T
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      ProfiBlot T48

      The ProfiBlot T48 is Tecan’s smart and reliable blot processor, designed to improve productivity and reproducibility by automating Southern blot and Western blot assays.

      It offers full automation of reagent dispensing, hybridization and washing of strip-based assays, and can be used for a range of applications, such as screening of infectious diseases, HLA-typing and HPV-testing.

      For reliable temperature control, the ProfiBlot T48 combines advanced top and bottom heating systems with an integrated reagent preheating station, as well as an active cooling system.

      Built to improve safety and reliability in the laboratory, the ProfiBlot T48 has been designed to meet the IVD-directive 98/79/EC for Europe. It processes the entire assay in a disposable tray within an enclosed environment to minimize human exposure to potentially infectious samples.

      Product highlights include:

      • Advanced fluid-based system for active heating and cooling of the tray
      • Additional top heating element to avoid evaporation during run
      • Integrated reagent preheating and magnetic stirrer system
      • Reagent saving feature to minimize dead volumes and running costs
      • Automated processing of up to 48 samples per run
      • Fast washing of three assay strips in parallel for high throughput
      • On-board operation and BlotWare™ software for easy programming
      • Auto-calibration of dispense volumes eliminates tedious manual procedure
      • Predefined cleaning protocols for easy maintenance
      Show more

      Specifications

      General
           
      Display unit Liquid crystal display with 2 rows of 16 digits
      Keyboard 4-key membrane keyboard
      Number of dispensing channels 6 (optionally 7)
      Capacity of reagent bottles 4 (optionally 5) x 250 ml, 1 x 500 ml, 1 x 1000 ml
      Capacity of waste bottles 1 x 500 ml toxic waste, 1 x 2500 ml biohazardous waste
      Capacity of bottles for heating system 1 x 2000 ml, 1 x 3000 ml
      Computer interface USB, RS232
      Incubation temperature adjustable between 35 °C and 65 °C
      Temperature accuracy ± 1 °C
      Volume of reagent dispensed 250 – 3000 µl in 250 µl steps
      Dispensing accuracy 2000 µl: less than or equal to 10%
      250 µl: +25% to -20%
      Cooling Temperature < 30 °C within 6 min
      Power
           
      Supply Auto-sensing for the settings
      100 – 120 V and 220 – 240 V
      50 or 60 Hz
      Main fuses 2 x T 8A / 250V (Slow blow)
      Consumption Max. 900 VA
      Physical
           
      Width closed/ opened 804 mm / 804 mm
      Height closed/ opened 460 mm / 671.4 mm
      Depth closed/ opened 459 mm / 510.6 mm
      Weight 47 kg

      Literature

      Brochure - ProfiBlot T48

      The ProfiBlot 48 is an automated Western Blot & Southern Blot Processor for up 48 samples per run.
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