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Tecan’s family of blot processors support automated washing and incubation of up to 48 assay strips per run. It includes the temperature controlled ProfiBlot T48, which takes care of hybridization and stringent wash steps, enabling walkaway processing of Southern blot assay strips. In addition, the ProfiBlot 48 takes care of non-heated incubation and washing steps for automated processing of Western blot assays strips.
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Tab 01 / Overview
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The ProfiBlot 48 and ProfiBlot T48 blot processors are easy to operate, and can be programmed either from a PC using the BlotWare™ software, or by using the integrated display and keypad. Dispense volumes, selection of dispense channels, shaking speed for incubation steps and wash parameters can be arranged in any combination within a protocol, and up to 20 different methods can be stored. Once programming and downloading are completed, the PC can be disconnected and the ProfiBlot will perform the assays in standalone mode.
After the user has placed the assay strips into the individual wells of the tray, the samples can be added using a pipette. At the push of a button, the entire procedure can be performed with no further input from the user. The display provides a way of checking every stage of the process, and an audible signal indicates when the assay is complete.
For fast dispensing, the ProfiBlot processors have two reagent channels with three pumps working in parallel, and up to five channels for single position reagent addition. For fast aspiration of the wells, liquid is removed from the tray by using three aspiration pumps in parallel, processing three strips at a time. This fast method of dispensing and aspiration ensures reproducible timing, even for kits requiring short incubation times. Additionally, an automated cleaning procedure simplifies daily maintenance at the push of a button.
Load up to 48 assay strips into the individual wells of the tray, add the samples and press start.
The ProfiBlot 48 and T48 instruments use disposable trays only, which are designed to meet the IVD-directive 98/79/EC and eliminate the need for tedious cleaning of the sample holders. This is advantageous over reusable sample holders, which are often difficult to clean due to the background color of the tray material, reducing chances of false positive results and a need for retesting.
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Tab 02 / ProfiBlot 48
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The ProfiBlot 48 provides reliability and increased productivity by automating all key protocol steps of Western blot processing, including time-consuming washing and incubation procedures.
For optimum throughput, the ProfiBlot 48 processes up to 48 samples per run and supports a range of Western blot applications, such as for the confirmation of infectious and autoimmune diseases, as well as for allergy screening.
The ProfiBlot 48 has been designed to improve safety and reliability in the laboratory, and to meet the IVD-directive 98/79/EC for Europe. It processes the entire assay in a disposable tray within an enclosed environment to minimize human exposure to potentially infectious samples.
Product highlights include:
| Display unit | Liquid crystal display with 2 rows of 16 digits | |
| Keyboard | Four key membrane keyboard | |
| Number of dispensing channels | 6 (optional 7) | |
| Capacity of reagent bottles | 5 (optional 6) x 250 ml, 1 x 1000 ml | |
| Capacity of waste bottles | 1 x 500 ml toxic waste , 1 x 2500 ml hazardous waste | |
| Program editor software | Blotware™ | |
| Computer interface | USB, RS232 | |
| Disposable 48 well tray | Black, antistatic | |
| No. of strips to be processed | Up to 48 strips | |
| No. of processing steps for each program | Up to 59 different steps in one program | |
| No. of programs | Up to 20 different programs | |
| Dispense volume | 250 µl to 3000 µl in 250 µl steps | |
| Incubation time | 1 – 999 min in 1 min steps | |
| Dispensing accuracy | @2000 µl: less than or equal to 10 % @250 µl: +25% to -20% |
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| Width closed / openend | 804 mm / 804 mm | |
| Height closed / openend | 460 mm / 671.4 mm | |
| Depth closed/ opened | 459 mm/ 510.6 mm | |
| Weight | 36.8 kg | |
| Supply | Auto-sensing for the settings 100 – 120 V and 220 – 240 V 50 or 60 Hz |
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| Main fuses | 2 x T1 A / 250V (Slow Blow) | |
| Consumption | Max. 85 VA | |
The ProfiBlot 48 is an automated Western Blot Processor for up 48 samples per run.
User story with Mikrogen, explaining how the ProfiBlot 48 can be used for a strip-based Western Blot assay.
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Tab 03 / ProfiBlot 48T
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The ProfiBlot T48 is Tecan’s smart and reliable blot processor, designed to improve productivity and reproducibility by automating Southern blot and Western blot assays.
It offers full automation of reagent dispensing, hybridization and washing of strip-based assays, and can be used for a range of applications, such as screening of infectious diseases, HLA-typing and HPV-testing.
For reliable temperature control, the ProfiBlot T48 combines advanced top and bottom heating systems with an integrated reagent preheating station, as well as an active cooling system.
Built to improve safety and reliability in the laboratory, the ProfiBlot T48 has been designed to meet the IVD-directive 98/79/EC for Europe. It processes the entire assay in a disposable tray within an enclosed environment to minimize human exposure to potentially infectious samples.
Product highlights include:
| Display unit | Liquid crystal display with 2 rows of 16 digits | |
| Keyboard | 4-key membrane keyboard | |
| Number of dispensing channels | 6 (optionally 7) | |
| Capacity of reagent bottles | 4 (optionally 5) x 250 ml, 1 x 500 ml, 1 x 1000 ml | |
| Capacity of waste bottles | 1 x 500 ml toxic waste, 1 x 2500 ml biohazardous waste | |
| Capacity of bottles for heating system | 1 x 2000 ml, 1 x 3000 ml | |
| Computer interface | USB, RS232 | |
| Incubation temperature | adjustable between 35 °C and 65 °C | |
| Temperature accuracy | ± 1 °C | |
| Volume of reagent dispensed | 250 – 3000 µl in 250 µl steps | |
| Dispensing accuracy | 2000 µl: less than or equal to 10% 250 µl: +25% to -20% |
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| Cooling | Temperature < 30 °C within 6 min | |
| Supply | Auto-sensing for the settings 100 – 120 V and 220 – 240 V 50 or 60 Hz |
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| Main fuses | 2 x T 8A / 250V (Slow blow) | |
| Consumption | Max. 900 VA | |
| Width closed/ opened | 804 mm / 804 mm | |
| Height closed/ opened | 460 mm / 671.4 mm | |
| Depth closed/ opened | 459 mm / 510.6 mm | |
| Weight | 47 kg | |
The ProfiBlot 48 is an automated Western Blot & Southern Blot Processor for up 48 samples per run.