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      Resolvex®

      Smart sample preparation

      Sample preparation continues to be the most time-consuming part of an analytical workflow. Tecan’s range of smart consumables, coupled with its automation expertise, can help improve the throughput and quality of your sample preparation workflow.
      Smart Consumables
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      Tab 01 / Overview
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      Tab 02 / Smart Consumables
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      Cerex® and Narrow Bore Extraction™ (NBE™) smart consumables

      Solid phase extraction consumables

      Tecan's suite of microparticulate solid phase extraction (SPE) consumables uses microparticulate sorbents with optimized small flow paths, to ensure greater contact with the analyte molecules. This enables better clean-up of the sample matrix, leading to cleaner sample extracts.

      Tecan also offers a variety of sorbent chemistries, from state-of-the-art polymer technologies to unique target resins for more complex workflows.

      Why microparticulate polymeric materials?

      Capture efficiency refers to the ability of an SPE device to effectively retain or ‘trap’ desired analytes from the sample. SPE products that use large particle sorbents may exhibit significant analyte breakthrough during sample application, regardless of the chemical affinity of the sorbent for the analyte species. This is caused by the relatively large flow paths between the sorbent particles, which allow analytes to bypass contact with the sorbent and avoid retention (Figure 1).

      In contrast, Cerex microparticulate sorbents have much smaller flow paths, ensuring contact with the analyte molecules passing through the sorbent bed.

      Figure 1: 40-micron path


      Figure 2: 10-micron path

      Microparticulate polymeric materials allow for fast and efficient binding of analytes, resulting in smaller sorbent bed sizes

      The difference between using 8 μm sorbent and conventional 40 μm sorbents of surface-matched chemistries was demonstrated by performing a loading experiment in an online cartridge format, measuring the breakthrough of a UV-absorbing analyte from each cartridge using a UV detector (Figure 3).

      1. Initially, the cartridge is bypassed; the detector signal reflects the level of analyte in the loading solvent.
      2. When the cartridge is placed into the sample stream, the absorbance initially drops and then recovers as analyte breakthrough occurs.
      3. The 8 µm cartridge captures roughly four times the analyte quantity compared to the 40 μm cartridge before breakthrough occurs, reaching total saturation immediately afterwards, as shown by the rapid recovery of the detector signal to initial conditions.
      4. In contrast, the 40 μm cartridge never reaches saturation, even after a significant period of time.

      Figure 3: Loading experiment

      Figure: Loading experiment

      Loading time: time required to bind analyte to column material

      Microparticulate sorbents

      Figure 1: 40-micron path

      Most competitors

      • Large flow paths
      • Reduced sorbent contact
      • Analyte breakthrough possible

      Figure 2: 10-micron path

      Tecan SP

      • Much shorter flow paths
      • Increased sorbent contact
      • Improved loading
      • No breakthrough – robust, efficient and fast!

       

      The Van Deemter equation applies to chromatographic separations

      To obtain high resolution, increase the number of HETP (Height Equivalent to a Theoretical Plate) by decreasing the particle size of the sorbents as shown here.

      Microparticulate polymeric material provides smaller elution volume

      The high capture efficiency of microparticle sorbents allows much smaller bed sizes to be used in Cerex columns compared to conventional sorbents; a 20 mg Cerex column typically exhibits the same effective capacity as a 100 mg, 40 μm conventional SPE product.

      The use of much smaller sorbent beds allows for the use of smaller analyte elution volumes too, as demonstrated in Figure 4.

      Figure 4:
      Efficient elution with microparticulate sorbents

      Efficient Elution with Micro-Sorbents

      Curtesy of Spark Holland

      Cerex columns
      patented and proven technology


      Narrow Bore Extraction (NBE) technology offers higher performance than standard SPE

        Monodispersed particle size for maximum efficiency
        Less elution volume required
        Decoupled upper frit design retains maximum filtration capacity
        Air gap controls liquid flow for incubation workflows

      NBE – next generation SPE

      What is NBE good for?

      • Delivers fundamental redesign of SPE workflows with substantial improvements
      • Novel ‘Pocket Seal’ prevents sample flow until positive pressure is applied, allowing extended incubation steps on column and automation
      • Narrower particle size distribution improves extraction efficiency
      • Hot spot contamination eliminated
      • 5x smaller elution volumes allows injection into the MS instrument without concentration – just elute and shoot
      • Increased sensitivity and reproducibility allow use of less sensitive LC-MS equipment, reducing costs
      • Customers can reduce sample size, resulting in cost savings and simplification of the overall workflow

      NBE and Controlled Flow Plate (CFP) condense SPE workflows

      Resolvex_Smart_Consumables_10_720x480

       

      Upper bed provides a barrier to liquid flow and filtration during pressure processing steps:

      • Upper chamber can be used as a reaction vessel
      • Liquid is retained until 15 psi of positive pressure is applied

       

      1. Air lock between the solid barriers prevents the flow of liquid from the upper chamber
      2. Monodisperse microparticulate bed with selected chemistry
      3. Proprietary ‘Inactive Chemistry Bed’ minimizes interaction of target analytes

      NBE columns allow consolidation of workflow steps

      NBE microparticulate columns deliver faster flow rates, lower elution volumes and reduced solvent consumption compared to classical SPE and supported liquid extraction (SLE).

      • New dual chamber design for high SPE performance
      • Microparticulate sorbent for high efficiency
      • Integration of pre-SPE steps in the reaction vessel
      • Filter for matrix clean-up
      • Air lock prevents dripping
      • Choice of sorbents for SPE or filtration




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      Tab 03 / Reagent Kits
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      Tab 04 / Positive Pressure Processors
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      Tab 05 / Benchtop Autom. Solutions
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      Tab 06 / Proteom. Sample Prep. Workflows
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      Tab 07 / Literature
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      Related products
      Narrow Bore Extraction consumables

      The Resolvex range has been optimized for use with our suite of Cerex® and NBE smart extraction consumables.

       
      Positive pressure workstations

      The Resolvex smart extraction consumables have been optimized to work with our suite of Resolvex positive pressure SPE workstations.

      Read more
      Tecan Journal articles

      Read what Tecan users have to say.

      The Blog articles

      Read our Tecan-related blog articles.
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